Fig. 1. The measurement of the cell death, ROS generation in N2A cells under high glucose condition and agmatine treatment. (A) The graph showed that glucose concentration dependent decrease of cell viability of N2A cell compared to the normal control group. The more concentration of glucose was added, the more cells were dead. Each experiment included the 6 repeats per condition. (B) The cells treated with both glucose 200 mM and agmatine were exhibited increase in cell viability compared to the same glucose stress. Each experiment included the 6 repeats per condition. (C) Western blotting experiments showed that the relative protein level of cleaved caspase 3 /caspase 3 significantly was reduced in the glucose with agmatine 200 mM treatment group compared to the glucose treatment group. Each experiment included 4 repeats per condition. (D) ROS levels of conditioned N2A cells were measured using DCF-DA. ROS levels in agamatine treatment group were almost the same with the normal control group. ROS levels in N2A cells were increased in high glucose injury compared to the normal control group. Under high glucose condition, ROS levels in the agmatine teatment group tend to attenuate compared to high glucose exposed group. Agmatine reduced the high glucose-induced increase in DCF-DA-positive cells (green). Each experiment included 4 repeats per condition. 2', 7'-dichlorodihydrofluorescein diacetate (DCF-DA): green, Scale bar=200 µm.
© Exp Neurobiol
