Fig. 4. The measurement of p21, p35, Cdk 5 level in N2A cells under high glucose condition and agmatine treatment. (A) p21 mRNA level was measured by using reverse transcription-PCR. In high glucose group, mRNA level of p21 was increased compared to the normal control group. The agmatine treatment group showed lesser mRNA levels of p21 in spite of the high glucose toxic condition. (B) p35 mRNA level was measured by using reverse transcription-PCR. In high glucose group, mRNA level of p35 was increased compared to the normal control group. The agmatine treatment group showed lesser mRNA level of Cyc p35 in spite of the high glucose toxic condition. (C) Cdk 5 mRNA level was measured by using reverse transcription-PCR. In high glucose group, mRNA level of Cdk 5 was increased compared to the normal control group. The agmatine treatment group showed lesser mRNA level of Cdk 5 in spite of the high glucose toxic condition. Data were expressed as mean±S.E.M, and each experiment included 3 repeats per condition. β-actin was used as an internal control. Differences were considered significant at *p<0.05, *p<0.001.
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