Fig. 3. Infection by AAV. (a) Immunostaining of NeuN (red in horizontal line), GFAP (red in vertical line). AAV-GFP was injected into the putamen region. The fluorescence is detected by confocal microscope imaging. Scale bar, 25 µm. (b) High magnification images of GFP expressing cell. GFP and NeuN are well colocalized in neuronal cell body. Scale bar, 5 µm. (c) Cell counting analysis for GFP expressing cells of colocalization with NeuN or GFAP. Approximately 80% of GFP expressing cells are colocalized with NeuN. Analysis is performed with ImageJ (n=206: total number of cells).
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