Fig. 3. The phosphatase inhibitor, pervanadate, rescues the level of pY397 in cells overexpressing T474E-FAK or G2019S-LRRK2. (A) T474E-FAK-overexpressing HEK293T cells were treated with indicated amounts of pervanadate (PV) for 1 h. (B) G2019S-LRRK2-overexpressing HEK293T cells were treated with100 µM ADP in the presence of PV. Western blot analysis was carried out using pY397-FAK-specific antibodies. FLAG and FAK were detected as loading controls.
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