Administration protocols of psychosis animal model of amphetamine, scopolamine, PCP/ketamine and LSD in rats and mice
DrugDoseDurationStrainBehavior testReference
AmphetamineSub-chronic and incremental dosage schedule3 injections (06:00,12:00, and 18:00)/day for 6 daysDay 1 – 1 mg/kg, 2 mg/kg, and 3 mg/kg90 dayWistar ratsAmphetamine sensitization (1 mg/kg challenge)[14]
Day 2 – 4 mg/kg, 5 mg/kg, and 5 mg/kg
Day 3-6 – 5 mg/kg, 5 mg/kg, and 5 mg/kg
3 injections (08:00,14:00, and 20:00)/day for 6 daysDay 1 – 1 mg/kg, 2 mg/kg, and 3 mg/kg28 dayWistar rat/MaleDisrupted latent inhibition[12]
Day 2 – 4 mg/kg, 5 mg/kg, and 5 mg/kg
Day 3–6 – 5 mg/kg, 5 mg/kg, and 5 mg/kg
Chronic and incremental dosage scheduleOnce daily for day, 3 times (Monday, Wednesday, and Friday) for a weekWeek 1 – 1 mg/kg22 daySprague – Dawley rat/MaleDisrupted PPI and amphetamine sensitization (3 mg/kg challenge)[2]
Week 2 – 2 mg/kg
Week 3 – 3 mg/kg
Once daily, 3 times (Monday, Wednesday, and Friday) for a weekWeek 1 – 1 mg/kg22 daySprague – Dawley rat/MaleDisrupted PPI and amphetamine sensitization (3 mg/kg challenge)[2]
Week 2 – 2 mg/kg
Week 3 – 3 mg/kg
Week 4 – 4 mg/kg
Week 5 – 5 mg/kg
ScopolamineAcute schedule0.15 and 0.5 mg/kg<1 dayWistar rat/MaleDisrupted latent inhibition[3]
0.3 and 0.5 mg/kg<1 dayC57BL/6J mouse/FemaleSocial recognition deficit in 3-chamber test[20]
0.3, 1, 2, and 3 mg/kg<1 dayCD-1 mouse/MaleWorking memory deficit (T-maze spontaneous alteration)[85]
10 mg/kg<1 dayC57BL/6NCrl mice/malePPI impairment[21]
KetamineAcute schedule100 mg/kg<1 daySwiss mouse/MaleHyperlocomotion and excessive fear (latency time of fear conditioning was increased)[4]
Sub-chronic scheduleOnce daily for 5 days10 mg/kg21 dayHooded Lister rat/MaleWorking memory deficit[35]
Once daily for 5 days30 mg/kg10 dayWistar rat/MaleHyperlocomotion[89]
Once daily for 5 days30 mg/kg21 dayHooded Lister rat/MaleIncreased immobility time in forced swim test[86]
2 injection for 6 days30 mg/kg10 dayLong Evans rat/MaleWorking memory deficit (Mismatch detection test)[89]
Chronic scheduleOnce daily for 10 days100 mg/kg11 daySwiss mouse/MaleHyperlocomotion, increased immobility time in forced swim test, and increased latency time of fear conditioning[4]
PCPAcute schedule5 mg/kg<1 daySprague–Dawley ratHyperlocomotion[32]
2.58 mg/kg<1 dayLong–Evans ratsAttentional set-shifting deficit (Extra dimensional shift)[33]
1.5 mg/kg<1 dayC57Bl/6J mouse/MaleHyperlocomotion, stereotype behavior, and reduced social interaction[87]
2 mg/kg<1 dayC57Bl/6J mouse/MaleHypolocomotion and reduced social interaction[87]
5 mg/kg<1 dayC57BL/6J mice/MaleHyperlocomotion[5]
Sub-chronic schedule2 injection (0800 2000) for 7 days5 mg/kg10 dayLong-Evans ratAttentional set-shifting deficit (Extra dimensional shift)[34]
Chronic scheduleOnce daily for 10 days (days 1~5, 8~12)5 mg/kg<1 dayC57BL/6J mice/MaleHyperlocomotion and disrupted working memory[5]
LSDAcute schedule0.03, 0.1, and 0.3 mg/kg<1 dayWistar Rats/MaleHyperlocomotion and disrupted PPI[43]
0.1 and 0.3 mg/kg<1 daySprague-Dawley RatsHyperlocomotion and disrupted PPI[43]
Chronic scheduleOnce daily and every other day for 90 days0.16 mg/kg1 monthSprague-Dawley rat/MaleHyperlocomotion, decreased social behavior, and anhedonia[6, 42]

aPCP, phencyclidine; LSD, lysergic acid diethylamide; PPI, prepulse inhibition.

This table includes the drug, administration protocol, time taken behavioral experiments after administration of the drug, strain, and psychotic behaviors that was determined by each administration protocol. The administration protocol for 10 days or less was marked as a sub-chronic schedule, and administration protocol for over 10 days was marked as a chronic schedule. In the case of behavioral testing on the day of drug administration, it was labeled as occurring for less than 1 day.

Exp Neurobiol 2017;26:11~24
© Exp Neurobiol