Fig. 3. LPS/FN-γ induced sequential expression of p-CTMP, p-Akt, CTMP and p-CREB in primary astrocytes. (A) Serum-starved astrocytes were treated with LPS/IFN-γ for the indicated time period, and western blot analysis was performed using anti-p-CTMP, anti-p-Akt (T308), anti-CTMP, and anti-p-CREB. Data are expressed as optical densities and represent means±SEM of three independent experiments (**p<0.01, ***p<0.001). (B) Mice were administrated with KA for 1 (B-B, F, J), 3 (B-C, G, K) and 7 days (B-D, H, L). In the control (B-A, E, I), few p-CREB were co-localized with GFAP in the CA3 region of the hippocampus. After KA treatment, p-CTMP/GFAP positive cells were markedly increased at 3 day (arrowheads) and then decreased. Nuclear counterstaining was performed with DAPI (blue). (Scale bar=20 µm).
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