Fig. 1. Polarization of BV2 microglial cells using conditioned media. (A) Schematic time course of the in vitro experiments. (B) Immunohistochemistry of CD68 (M1 phenotype microglia; green) and CD206 (M2 phenotype microglia; red). In BV2 microglial cell, the expression of CD68 significantly increased in M1 conditioned media compared with M2 conditioned media. CD206 is expressed only in M2 conditioned media (Scale bar=20 um, n=5/group).
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