Fig. 4. TGF-α is one of the major cytokines in M2 conditioned media. (A) RT-PCR of IL-10 and TGF-α in M0, M1, or M2 conditioned media. IL-10 and TGF-α expression is significantly increased at the mRNA level in M2 conditioned media. (B) The graph depicts the relative mRNA expression of IL-10 and TGF-α in M0, M1, and M2 conditioned media. The expression of these genes is significantly increased in M2 conditioned media. (C) The expression level of TGF-α in M0, M1, and M2 conditioned media using ELISA assay. The quantity of TGF-α is significantly increased in M2 conditioned media compared with M1 (***p<0.01 vs. M0 conditioned media, n=5/group).
© Exp Neurobiol
