Fig. 1. Immunohistochemical localization of TCTP in coronal sections of the mouse brain. Sections were stained by anti-TCTP antibody (a, b, d, e, f, and g) or anti-synapsin II antibody (c, h). Immunohistochemical signals were visualized using DAB and cell nuclei were stained with hematoxylin. Anti-synapsin II staining was used for staining of axonal tracts (c, h). Anti-TCTP staining was widely distributed in the brain, along with distinct localizations in axon tracts and axon terminals. A subset of axon fibers in the corpus callosum (CC) were moderately stained by anti-TCTP, while the internal capsule (IC) was absent from TCTP (d). Axonal fibers running in the subplate (SP) underneath the parietal cortex were stained by anti-TCTP (g, h). Red arrows in the parietal cortex indicate dendritic shafts arising from pyramidal neurons in the layers 3-5 heading towards the layer 1 (g, h). CC, corpus callosum; MHb, medial habenula; LHb, lateral habenula; Or, stratum oriens; Pyr, stratum pyramidale; DG, the dentate gyrus; SP, subplate; I-VI, cortical layers 1-VI. Scale bars: 200 µm (B, C, D, E, F), and 50 µm (G, H).
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