Fig. 2. Silibinin attenutes GCD through inhibition of mTORC1 pathway in the hippocampal DG by KA treatment in mice. (A, B) Morphologic analysis of dispersed granule cells 7 days after intrahippocampal KA injection. (A) The representative sections of the ipsilateral DG following Nissl staining by cresyl violet. CON, contralateral control side. Scale bar, 200 µm. (B) Morphologic analysis show that the quantification of GCD normalized to the contralateral side for each sample. *p<0.05 vs. PBS alone, #p<0.05 vs. KA alone (one-way ANOVA and Tukey's post-hoc analysis; n=4, each experimental group). All values are expressed as the mean±standard error of the mean (SEM). (C) Representative coronal sections of the hippocampal DG following p-4E-BP1 immunostaining 2 days after KA treatment in the hippocampus. (D, E) Western blot analysis of p-4E-BP1, 4E-BP1, p-p70S6K and p70S6K expression in the DG 2 days after KA treatment. The densities of p-4E-BP1 and p-p70S6K bands were normalized to the β-actin bands for each sample. *p<0.001 and **p<0.01 vs. PBS alone, #p<0.01 and ##p<0.05 vs. KA alone (one-way ANOVA and Tukey's post-hoc analysis; n=4, each experimental group). All values are expressed as the mean±SEM.
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