Fig. 4. Immunohistochemistry of nNOS and iNOS in the ischemic rat brain. (A) nNOS-positive cells (brown) in the ipsilateral brain were significantly decreased in the IP group compared with the EC group at 23 hr after reperfusion. (B) Quantification of nNOS-positive cells were analyzed by Image J. The nNOS-positive area in cortex and striatum was significantly decreased in the IP group compared to the EC group at 23 hr after reperfusion. (C) The expression of iNOS-positive cells (brown) in the IP group was significantly decreased in the cortex and striatum at 23 hr after reperfusion. (D) Quantification of nNOS-positive cells in the cortex was significantly different between the EC and IP groups, but not in the striatum at 23 hr after reperfusion. EC, Experimental control; IP, Ischemic preconditioning, Scale bar is 50 µm (***p<0.01 vs. EC).
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