Fig. 4. Validation of antibody using MOR shRNA. (A) Confocal images of MOR (green) after infection of lentivirus carrying MOR-shRNA-katushka (upper) or scrambled-shRNA-katushka (lower), respectively. (B) In vitro knockdown efficiency test of MOR shRNA in cultured hippocampal astrocytes by western blot. Upper band indicates MOR immunoactivity and lower band indicates actin immunoactivity. (C) In vivo knockdown efficiency test of MOR shRNA in CA1 hippocampus by western blot.
© Exp Neurobiol
