Fig. 6. Morin downregulates the expression of inflammatory mediators in hippocampus of KA-treated mice. Morin was orally administered to the mice 1 day before KA injection, and then administered for a further 2 days. (A~C) Double-immunofluorescence staining for Iba1 (red) and TNF-α (green), Iba1 (red) and IL-1β (green), and Iba1 (red) and iNOS (green) in the hippocampal CA1 regions. Scale bar, 20 µm, 5 µm. (D, E) Western blot analysis of TNF-α, IL-1β, and iNOS expression in the hippocampus. The densities of the TNF-α, IL-1β, and iNOS bands were normalized to the β-actin bands for each sample. *p<0.05 and ***p<0.001 vs. CON, #p<0.05 and ##p<0.01 vs. KA alone (one-way ANOVA and Tukey's post-hoc analysis; n=4, each experimental group). All values are expressed as the mean±SEM.
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