Fig. 1. Sequence analysis of targeted region of 14-3-3γ KO mice. (A) A genomic illustration of gene-trap strategy for 14-3-3γ KO mice. The organization of pU-21W trap-vector. EN2-intron, 1.2 kb of Mouse En2 intron 1; EN2-SA, splice acceptor of mouse En2 exon 2; β-geo, β-galactosidase; pA1, SV40 polyadenylation signal; pA2, mouse PGK gene polyadenylation signal; FRT, FLP recombinase target sequence. (B) The alignment of the mRNA sequences from RT-PCR of transgenic region and predicted protein sequence. (C) A map illustrates nine α-helical domains of WT of 14-3-3γ, and mutant 14-3-3γ from these transgenic mice only has 3 N-terminal domains (A~C).
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