Fig. 2. E-LTP is impaired in Rev-erbα KO mice during the subjective night. (A) The input-output relationships at SC-CA1 synapses showed no significant differences between WT and Rev-erbα KO mice at CT12~13. (WT, 12 slices from 7 mice; Rev-erbα KO, 12 slices from 7 mice; repeated-measures two-way ANOVA, effect of genotype, F(1,22)=0.5963; p=0.9631). (B) Paired-pulse ratios showed no significant differences between the WT and KO mice at CT12~13. (WT, 12 slices from 7 mice; Rev-erbα KO, 13 slices from 7 mice; repeated-measures two-way ANOVA, effect of genotype, F(1,22)=0.6569; p=0.7418). (C) Representative traces from baseline and LTP at times indicated by (a) and (b): WT (black) and KO (blue). TBS-induced E-LTP was significantly lower in the Rev-erbα KO mice (WT, 11 slices from 7 mice; Rev-erbα KO, 11 slices from 7 mice; average fEPSP slopes for the last 5 min; WT, 146.4%±6.073%; Rev-erbα KO, 128.7%±4.288%; unpaired t-test, p=0.0274). *p<0.05.
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