Fig. 5. The difference in histology with H&E staining and immunohistochemistry with PNA, Rhodopsin, PKCα, Brn3, GFAP, and TUNEL assay between the non-degenerated and degenerated areas at six weeks after intravitreal injection of 0.2 mg of MNU with vitrectomy. Histology with H&E staining showed not only the photoreceptor layer but also all layers of the retina appeared normal in the non-degenerated area (a), but loss of the photoreceptor layer was found in the degenerated area (b). In the non-degenerated area, cone and rod photoreceptor cells were normally expressed with PNA (c) and Rhodopsin staining (e), respectively. However, in the degenerated area, the cone photoreceptor cells were less expressed with PNA staining (d) and the rod photoreceptor cells were less expressed with Rhodopsin staining (f). Bipolar cells were well-expressed with PKCα staining (g, h) and ganglion cells were well-expressed with Brn3 staining (i, j) in both the non-degenerated (g, i) and degenerated areas (h, j) of the retina. Both the non-degenerated and degenerated areas showed an increase in GFAP staining (k, l), and there were no TUNEL-positive cells in either area (m, n). Next to all figures, three times enlarged figures (the part of dashed yellow box of a~n) are shown (a′~n′).
© Exp Neurobiol
