Fig. 1. UCH-L1 associates with lipid rafts. (A, B) SH-SY5Y cells (A) and rat primary neurons (B) were lysed in ice-cold 1% Triton X-100 buffer and fractionated as soluble or insoluble fractions, and then the samples were analyzed by Western blot. (C) After the treatment of SH-SY5Y cells with 10 mM MβCD for 1 h, the cells were lysed in ice-cold 1% Triton X-100 buffer and fractionated; the soluble and insoluble fractions were then analyzed by Western blot. Values obtained are from three independent experiments. *p<0.05, **p<0.01 against control by unpaired t-test. (D, E) SH-SY5Y (D) and rat primary neurons (E) were fractionated by sucrose gradient centrifugation fractionation assay in the presence or absence of 10 mM MβCD, and the lysates were then analyzed by Western blot.
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