Fig. 4. UCH-L1 regulates cell-to-cell transmission of α-synuclein. (A) NT and UCH-L1 KD #1 and #2 SH-SY5Y cells were co-cultured with differentiated α-synuclein overexpressing SH-SY5Y cells for 12 hr using dual chamber. (B) SH-SY5Y cells were treated with LDN-57444 for 24 h, and further co-cultured with differentiated α-synuclein overexpressing SH-SY5Y cells for 12 hr using dual chamber. The cells were then fixed and immunostained with the anti-α-synuclein antibody. The intensity was analyzed with the ImageJ program. Values are representative of three independent experiments. *p<0.05, **p<0.01 against control by one-way ANOVA (A) and unpaired t-test (B). (C) NT and UCH-L1 KD #1 and #2 SH-SY5Y cells were lysed. The lysates were then analyzed by Western blot. Values obtained are from three independent experiments.
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