Fig. 6. C3aRA treatment attenuates tight junction protein occludin and pERK1/2 expression in endothelial cells against OGD. A) Endothelial expression of occludin was observed by Western blot. B) Occludin densitometry analysis was done by Image J. C). pERK and ERK expression assessed by Western blot. D) ERK densitometry analysis was done by Image J software (NIH). The results represent the means±SEM of fold changes (n=4). *p<0.01, **p<0.001, ***p<0.0001. Cont, control group; OGD, ischemic group; Cont+C3aRA, drug control; OGD+C3aRA, drug treatment.
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