Fig. 4. Enhancement of immunoreactivity in FxClearprocessed tissue. (A) Reconstituted 3D images of brain slices. Slices were stained with Iba1, GFAP, Tyrosine-hydroxylase (TH), Calbindin, and DCX antibodies. Scale bar, 25 µm. (B) Comparison of fluorescence intensities from different tissue clearing processed groups. A graph showing the proportion of fluorescence intensity obtained with immunostained brain slices of three different group (n=7, each group). (C) Fluorescence intensity (left) and SNR (right) in FxClear-processed samples compared to PFA-only and ACT processed samples (n=6; *p<0.05, ***p<0.001).
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