Fig. 5. Correlation between phagocytic cells and pro/anti-inflammatory cytokines in the ischemic lesion. Paraffin-embedded brain sections were im-munostained with CD68 for phagocytic cells, TNFα/IL6 for pro-inflammatory cytokines, and IL10 for anti-inflammatory cytokines, and subsequently counterstained with DAPI for cell nuclei at one month after reperfusion. IL10-expressing cells were not correlated with phagocytosis (arrowheads). Scale bar represents 10 μm. The representative images were obtained from animal R330.
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