Fig. 2. Reduced activity and impaired motor coordination in Vps13b2−/− mouse. (A) Distance moved measured in 5-min intervals across the 30-min test session in open field test (WT: n=9 mice; Vps13b2−/−: n=12 mice; two-way ANOVA, genotype x time, F5,95=0.23, p=0.948; effect of genotype, F1,95=5.04, p=0.037; effect of time, F5,95=18.30, p<0.0001). (B) Total distance moved in open field test (WT: 10940±685.0 cm, n=9 mice; Vps13b2−/−: 9083±452.4 cm, n=12 mice; unpaired t-test, *p<0.05). (C) Time spent in peripheral (40 cm) and center (20 cm, 10 cm) zones in open field test (WT: n=9 mice; Vps13b2−/−: n=12 mice; two-way ANOVA, genotype x zone, F2,38=0.01, p=0.990; effect of genotype, F1,38=0.23, p=0.639). (D) Upper, Elevated zero maze apparatus. Lower, Time spent in closed and open compartment (WT: n=9 mice; Vps13b2−/−: n=12 mice; two-way ANOVA, genotype x sector, F1,19=0.42, p=0.524; effect of genotype, F1,19=1.86, p=0.189; effect of sector, F1,19=54.49, p<0.0001). (E) Upper, Light dark box test apparatus. Lower, Time spent in lit area (WT: 54.98±15.04 s, n=9 mice; Vps13b2−/−: 32.79±10.29 s, n=12 mice; unpaired t-test, p=0.223). (F) Upper, Rotarod test apparatus. Lower, Latency to fall in accelerating (4 to 40 rpm across 5 min) rotarod test (WT: n=9 mice; Vps13b2−/−: n=9 mice; unpaired t-test, *p<0.05). Latency measured in three repeated trials were averaged. Data were represented as mean±SEM.
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