Fig. 5. Effect of Tat-AR protein against H2O2-induced p53, Bcl-2, Bax, and Caspase-3 expression in HT-22 cells. One-hour pretreatment of HT-22 cells with Tat-AR protein (0.5~5 μM) or AR protein was followed with treatment with H2O2 (1 mM) for 20 min (p53), 90 min (Bcl-2), 120 min (Bax), and 6 h (Caspase-3), respectively. The expression levels of p53 (A), Bcl-2 and Bax (B), and Caspase-3 (C) were determined by Western blot analysis and the band intensity was measured by densitometer. *p<0.05 and **p<0.01 compared with H2O2-treated cells. Data are repressed as mean±SEM (n=3).
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