Lists of antibodies used for immunostaining and primers used for qPCR

MethodAntibodySpeciesDilutionCompanyCat. no.
Immunocytochemistry/immunohistochemistryHuman nestinRabbit1/200MilliporeABD69
GFAPRabbit1/1500DAKOZ0334
Tuj1Mouse1/200CovanceMMS-435-P
O4Mouse1/200MilliporeMAB345
PDGFRRabbit1/100Santa CruzSC-338
GDNFRabbit1/50Santa CruzSC-328
ImmunohistochemistryKu80Rabbit1/200Cell Signaling2180
STEM121Mouse1/500ClontechY40410
STEM101Mouse1/100ClontechY40400
Human nucleiMouse1/50ChemiconMAB1281
Olig2Rabbit1/200MilliporeAb9610
S100BGoat1/50R&D SystemsAF1820
Neurofilament LRabbit1/1500ChemiconAB9568
Neurofilament MRabbit1/1500ChemiconAB1987
Neurofilament HRabbit1/1500ChemiconAB1991
MBPRabbit1/400DAKOA0623
Schwann/2EMouse1/3000Cosmo BioCAG-Gu01-M01AS-A
GABARabbit1/500Sigma2052
Gene (protein)Amplicon length (bp)Primer sequence
SCN3A (Nav1.3)158F: 5′-AACGAAAGACGATCAAGACC-3′R: 5′-CCAAAGAAACATCAACGATCAG-3′
SCN9A (Nav1.7)163F: 5′-GGGAACTTGATCTTTACAGGG-3′R: 5′-ACTGATAATCCTTCCACATCTG-3′
18S rRNA77F: 5′-TTGATTAAGTCCCTGCCCTTT-3′R: 5′-CGATCCGAGGGCCTCACTA-3′
Exp Neurobiol 2019;28:679~696 https://doi.org/10.5607/en.2019.28.6.679
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