Fig. 4. Transplantation of GDNF-hNSPCs promotes motosensory recovery, reduces expression of voltage-gated sodium channels and NPY, and increases expression of GABA. (A) The BBB open-field walking scores before transplantation and at various time points post-transplantation. (B) Von Frey tests for mechanical allodynia in lesioned hindlimbs of rats with SCI before transplantation and at various time points post-transplantation. (C) qRT-PCR analysis of Nav1.3 and Nav1.9 expression in the T8~T10 segment of the spinal cord. (D, E) Representative images and quantification of NPY expression in lamina I of the dorsal horn in the T8 segment of the spinal cord. (F, G) Representative images and quantification of GABA expression in the T8~T10 segment of the spinal cord. (H) Many engrafted hNuc+ GDNF-hNSPCs (red, arrowheads) express GABA (green, arrowheads) adjacent endogenous GABA+ cells (green, arrows). Scale bars: 200 μm in D; 500 μm in F; and 50 μm in H. Data represent the means±SEM. *p<0.05 vs. vehicle, **p<0.01 vs. vehicle, ***p<0.001 vs. vehicle, #p<0.05 vs. Mock-hNSPCs, ##p<0.01 vs. Mock-hNSPCs in A, B. *p<0.05 vs. vehicle, #p<0.05 vs. Mock-hNSPCs in C, E, and G.
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