Fig. 6. Distribution and mRNA expression patterns of Tac1, ChAT, and Tacr1. (A) ISH images were acquired by serial sections (14 μm thick). The total number of sections were 88~90 and 75~80 in PBS and LPS groups, respectively. The images of the serial sections were stacked using the Image J program. Stacked images were reconstructed using the Amira program. (B) Each gene is expressed in a region-specific pattern; Tac and ChAT were enriched in dorsal and ventral portions of MHb, respectively, and Tacr1 was expressed in MHb and LHb. (C) For quantitative analysis, mRNA dot counting was performed on 10× magnified photographs.
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