Representative images indicating the non-overlapping expression of NG2 glia and either astrocytes or mature oligodendrocytes in the hippocampal CA1 region of rats at day 3 after transient forebrain ischemia. (A, B) Double-labeling for NG2 and GFAP in the pyramidal cell layer (PCL) and stratum radiatum (SR) of the ischemic CA1 hippocampus showing that reactive NG2 glia (arrowheads) and reactive astrocytes (arrows) have non-overlapping distributions. The boxed area in A is enlarged in B. (C, D) Double-labeling for NG2 and APC in the ischemic CA1 hippocampus showing that NG2 glia (arrowheads) are distinct from mature oligodendrocytes (arrows). The boxed area in c is enlarged in D. Cell nuclei are stained with DAPI. Scale bars represent 20 μm in A and C, 10 μm in B and D.