M2 promoted EpSC differentiation by upregulating SIRT2 expression. SIRT2 expression in the EpSCs cocultured with M1, M2 or M0 was detected by RT-PCR (a) and western blots (b). SIRT2 and β III-tubulin expression in the EpSCs from the WT mice cocultured with M1, M2 or M0 was detected by immunofluorescence (c). (Blue: DAPI; green: SIRT2; red: β III-tubulin; ×400). Scale bars: 25 µm. RT-PCR (d) and western blotting assays (e) were used to detect temporal SIRT2 expression in the EpSCs cocultured with M2 macrophages. Data represent three independent experiments. *p<0.05, **p<0.01, ***p<0.001.