Increases in GFRα-1 expression in the AAV1-Rheb(S16H)-treated hippocampus. (A) Representative images of immunostaining for GFRα-1. Immunohistochemical staining results showed the increases in GFRα-1 expression both in neurons (arrow heads) and non-neuronal cells (arrows) in the Rheb(S16H)-expressing hippocampus, as demonstrated by the magnified photomicrographs of the insets in the CA1 layer. Scale bars: upper images, 100 μm; lower images (insets), 20 μm. (B) Double immunofluorescence staining to visualize co-expression patterns of GFRα-1 and NeuN, or GFRα-1 and GFAP in the Rheb(S16H)-transduced hippocampus. Scale bars: images in first three columns from left, 50 μm; images in far-right column (insets), 20 μm. (C) Western blot analysis for GFRα-1 expression in the controls, AAV1-GFP- and AAV1-Rheb(S16H)-injected hippocampus of rat brains. Differences among groups were evaluated by one-way analysis of variance and Tukey’s post-hoc analysis; (*p<0.01
