Fig. 1. Experimental scheme for safe, fast and easy human tissue sampling, total RNA extraction and qRT-PCR. (A and B) Samples are collected by self-pharyngeal swab and RNA are extracted by (A) Trizol-based manual RNA extraction method (Pharyn/Manual) or (B) Trizol and commercial RNA extraction kit (Pharyn/Kit). Trizol is used for inactivating viral infection. (C and D) Samples are collected by self-saliva sampling and RNA are extracted by Trizol and commercial RNA extraction kit. Trizol and proteinase K is used for inactivating viral infection. (C) cDNA synthesis and qRT-PCR are separately performed from saliva sample (Saliva/Kit) (D) cDNA synthesis and qRT-PCR are combined in one reaction (Saliva/1-step kit).
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