Fig. 5. Effects of thyroxine on construct validity after chronic exogenous intraperitoneal injection in rats. (A, B) Effect of chronic elevation of thyroxine on the expression of melatonin receptor in hypothalamus of experimental rats. Immunofluorescence assay of MT (green) and DAPI (blue) expression in hypothalamus. Scale bar: 50 μm. (C) Distribution of corticosterone in the structure of functional brain regions. (D) Changes of 5-HT concentration in different brain regions. (E) Changes of Glu concentration in different brain regions. (F) Changes of GABA concentration in different brain regions. Data are represented as means±SEM, *p<0.05, **p<0.01, ***p<0.001 were considered to indicate significant difference between Control and Model. MT, melatonin receptor; DAPI, 4’,6-diamidino-2-phenylindole; 5-HT, 5-hydroxytryptamine; Glu, glutamic acid; GABA, γ-aminobutyric acid.
© Exp Neurobiol
