List of antibodies and reagents used for immunohistochemistry
| Target antigen | Host | Dilution | Cat number | Manufacturer | |
|---|---|---|---|---|---|
| Primary antibody | Iba1 | Rabbit | 1:1,000 | 019-19741 | Wako |
| Pu.1 | Rabbit | 1:400 | 2258S | Cell Signalling | |
| pH3 | Rabbit | 1:3,000 | 06-570 | Millipore | |
| MPO | Rabbit | 1:100 | 9535 | Abcam | |
| CD68 | Rat | 1:1,000 | MAC 1957 | BIO-RAD | |
| TREM2 | Sheep | 1:200 | F1729 | R&D Systems | |
| GFAP | Mouse | 1:100 | G3893 | Sigma-Aldrich | |
| IL-10R | Rabbit | 1:50 | Sc-985 | Santa Cruz | |
| CD11b | Rat | 1:100 | MCA711G | AbD Serotec | |
| CD3 | Hamster | 1:250 | MCA2690 | AbD Serotec | |
| Secondary antibody | Biotinylated | Rabbit | 1:500 | BA-1000 | Vector Laboratories |
| Biotinylated | Rat | 1:500 | BA-4001 | Vector Laboratories | |
| Biotinylated | Sheep | 1:500 | BA-6000 | Vector Laboratories | |
| Biotinylated | Hamster | 1:500 | BA-9100 | Vector Laboratories | |
| Biotinylated | Mouse | 1:500 | BA-2001 | Vector Laboratories | |
| Alexa 488 | Mouse | 1:500 | A11029 | Invitrogen | |
| Alexa555 | Rabbit | 1:500 | A21428 | Invitrogen | |
| Streptavidin –HRP | 1:500 | SA-5004 | Vector Laboratories | ||
| Streptavidin-Alexa Fluor 555 | 1:500 | S21381 | Life Technologies | ||
| Streptavidin-Alexa Fluor 488 | 1:500 | S11223 | Invitrogen | ||
| DAPI | 1:10,000 | 9542 | Sigma -Aldrich |