Fig. 1. Expressions of LRRK2 and phosphorylation of LRRK2 were induced in RB photothrombosis model. (A) Double immunofluorescence staining with LRRK2 (green) and the astrocyte marker GFAP (red) of the damaged cortex of mice at 3 days after Rose-Bengal photothrombotic stroke. The cells which have LRRK2-positive puncta and high expressed LRRK2 were observed in penumbra not infarct core. (B) Expression and localization of phospho-LRRK2 in mouse cortex cells. Double immunofluorescence staining with phospho-LRRK2 (green) and the neuronal cell marker NeuN (red) of the damaged cortex of mice at 3 days after Rose-Bengal photothrombotic stroke. The autophosphorylation site S1292 has been proposed as a physiological and direct marker of LRRK2 kinase activity. Scale bar=50 μm in i,ii,iii, 25 μm in a, b.
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