Highlights
  • Special Topic | April 30, 2020

    In response to the desperate need for a low-cost, easy-access, laboratory-ready, laboratory-safe protocol for an ultra-sensitive detection of SARS-CoV2, which has caused a world-wide outbreak of Coronavirus Disease 2019, we have established a real-time reverse-transcription polymerase chain reaction (rtPCR)-based assay protocol composed of easy specimen collection from a subject via pharyngeal swab, Trizol-based RNA purification, and SYBRGreen-based rtPCR. This protocol shows an accuracy and sensitivity limit of 1~10 virus particles as we tested with a known Lentivirus. The cost for each sample is estimated to be less than 15 US Dollars with overall time it takes for an entire protocol of less than 4 hours.

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    In response to the desperate need for a low-cost, easy-access, laboratory-ready, laboratory-safe protocol for an ultra-sensitive detection of SARS-CoV2, which has caused a world-wide outbreak of Coronavirus Disease 2019, we have established a real-time reverse-transcription polymerase chain reaction (rtPCR)-based assay protocol composed of easy specimen collection from a subject via pharyngeal swab, Trizol-based RNA purification, and SYBRGreen-based rtPCR. This protocol shows an accuracy and sensitivity limit of 1~10 virus particles as we tested with a known Lentivirus. The cost for each sample is estimated to be less than 15 US Dollars with overall time it takes for an entire protocol of less than 4 hours.
    Joungha Won, Solji Lee, Myungsun Park et al.
  • Review Article | April 30, 2020

    Phytoncides including monoterpene of the pine tree oils show sleep-enhancing effects by acting as a positive modulator for GABAA-BZD receptor.

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    Phytoncides including monoterpene of the pine tree oils show sleep-enhancing effects by acting as a positive modulator for GABAA-BZD receptor.
    Junsung Woo and C. Justin Lee
  • Short Communication | April 30, 2020

    Intracerebral transplantation of BDNF-overexpressing human neural stem cells (HB1.F3.BDNF) can survive, migrate, differentiate into DARPP-32-positive neurons, and finally promote functional improvement in the QA-lesioned rodent model of Huntington’s disease (HD), providing the possibility of using these cells to treat HD patients in the future.

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    Intracerebral transplantation of BDNF-overexpressing human neural stem cells (HB1.F3.BDNF) can survive, migrate, differentiate into DARPP-32-positive neurons, and finally promote functional improvement in the QA-lesioned rodent model of Huntington’s disease (HD), providing the possibility of using these cells to treat HD patients in the future.
    Hyun Sook Kim, Iksoo Jeon, Jeong-Eun Noh et al.
  • Original Article | April 30, 2020

    The present study demonstrates that neuronal transduction with Rheb(S16H) stimulates adult neurons to produce BDNF and GDNF by themselves, and the increases in those factors can activate astrocytes in the hippocampus in vivo, resulting in additional GDNF production. These observations suggest that Rheb(S16H) transduction of hippocampal neurons can construct a potential neuroprotective system through functional interactions between neurons and astrocytes, and that the system following Rheb(S16H) transduction may contribute to neuroprotection in the adult hippocampus in vivo.

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    The present study demonstrates that neuronal transduction with Rheb(S16H) stimulates adult neurons to produce BDNF and GDNF by themselves, and the increases in those factors can activate astrocytes in the hippocampus in vivo, resulting in additional GDNF production. These observations suggest that Rheb(S16H) transduction of hippocampal neurons can construct a potential neuroprotective system through functional interactions between neurons and astrocytes, and that the system following Rheb(S16H) transduction may contribute to neuroprotection in the adult hippocampus in vivo.
    Dongyoung Yun, Min-Tae Jeon, Hyung-Jun Kim et al.
Vol.29 No.2 | April 30, 2020

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Schematic diagram of the low-cost, laboratory-safe protocol for SARS-CoV2.

We propose a cost-effective, quick-and-easy method for early detection of SARS-CoV-2 at any conventional Biosafety Level II laboratories that are equipped with a rtPCR machine.

In response to the desperate need for a low-cost, easy-access, laboratory-ready, laboratory-safe protocol for an ultra-sensitive detection of SARS-CoV2, which has caused a world-wide outbreak of Coronavirus Disease 2019, we have established a real-time reverse-transcription polymerase chain reaction (rtPCR)-based assay protocol composed of easy specimen collection from a subject via pharyngeal swab, Trizol-based RNA purification, and SYBRGreen-based rtPCR. This protocol shows an accuracy and sensitivity limit of 1~10 virus particles as we tested with a known Lentivirus. The cost for each sample is estimated to be less than 15 US Dollars with overall time it takes for an entire protocol of less than 4 hours.