Fig. 3. Git1-/- mice show altered gait and impaired motor learning and coordination. (A) Representative images of gait pattern of wild-type littermates and Git1-/- mice in the catwalk analysis. n=8 (WT), 10 (KO). (B) Step patterns of Git1-/- mice are not different from those of wild-type littermates. Detailed description of each pattern is provided in the materials and method section. (C) Git1-/- mice show high percentage of regularity index patterns comparable to those of wild-type littermates. (D) Print position of right and left paws is significantly altered in Git1-/- mice. Git1-/- mice place hind paws up to the almost same previous position of their front paws, while wild-type littermate place hind paws on the slightly different position. (E) Base of support of both front and hind paws is significantly decreased in Git1-/- mice. (F) Stride length, the distance between consecutive placements of the same paw, of Git1-/- mice is significantly increased. RF, right front paw; LF, left front paw; RH, right hind paw; LH, left hind paw. (G) Git1-/- mice step on the ground more briefly with their paws than wild-type littermates, as shown in decreased duty cycle. (H) Impaired motor coordination and learning of Git1-/- mice in the accelerating rotarod test. n=8 (WT), 9 (KO). Repeated Measurements of ANOVA; Repeated Measurements of ANOVA; Main effect of genotype p<0.001, F(1,48)=117.185; Main effect of time p=0.050, F(3,48)=2.753; Main effect of time x genotype p=0.187, F(3,48)=1.653 *p<0.05, **p<0.01, ***p<0.001; Student's t test and repeated-measures ANOVA. Error bars indicate means±s.e.m.
© Exp Neurobiol
