Fig. 1. ATP release from astrocyte by mechanical stimulation. (A) Schematic illustration of sniffer-patch technique stimulated by pressure application of GPCR agonists and mechanical stimulation. Right pipette, recording from HEK293T cell expressing P2X2-V343Q. (B) Pseudo color images from Fura2-loaded astrocyte before and after mechanical stimulation. (Ca, Cb) Representative traces recorded from sniffer-patch experiment. Upper trace, Ca2+ transient by mechanical stimulation recorded from astrocyte. Lower trace, whole-cell current by mechanical stimulation recorded from HEK293T cell expressing P2X2-V343Q. Diamond, mechanical stimulation. Inset box, Full activation current recorded from HEK293T cell by bath application of 100 µM ATP.
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