Fig. 4. Ca²⁺ entry is critical for MS-induced ATP release from astrocyte. (A, B) Representative trace of sequential recording of Ca²⁺ and ATP release in same astrocyte and summary bar graph of 1^st/2^nd ratio for the response of Ca²⁺ and ATP amplitude. (C, D) Representative traces recorded from Fura2 imaging and sniffer-patch experiment in Ca²⁺ free condition (C) and Ca²⁺ free with TG condition (D). Diamond indicates the timing of mechanical stimulation. (E) Summary bar graph showing the ratio of Ca²⁺ response with Ca²⁺, Ca²⁺ free and Ca²⁺ free + TC in external solution induced by mechanical stimulation. Paired two-tailed t-test (^**p<0.01, ^***p<0.001). (F) Schematic diagram of pathway of ATP release compared with mechanical stimulation v.s. GPCR activation.

Exp Neurobiol 2015;24:17~23 https://doi.org/10.5607/en.2015.24.1.17

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