Fig. 2. Effect of i.c.v. pretreatment with KA on the plasma corticosterone (A) and insulin levels (B) in D-glucose-fed model. After mice were pretreated i.c.v. with KA (0.1 µg) for 24 h, they were fed orally with D-glucose (2 g/kg). The plasma corticosterone and insulin levels were measured before and 30 min after D-glucose feeding. The vertical bars indicate the standard error of mean (***p <0.001, **p <0.01, *p <0.05; compared to PBS+Saline, +++p <0.001; compared to PBS+D-glucose). The number of animals used for each group was 8~10.
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