Fig. 2. Immunochemical image for measurement of AQP-1 expression by DHA treatment. (A) Immunochemical images showed that in DHA treatment group, AQP-1 positive cells (green) was decreased in rat cortex, compared with the EC group. (B) In DHA treated ischemic striatum, AQP-1's expression was decreased in striatum owing to DHA treatment. (C) The graph showed the percentage (%) of AQP-1 positive cells to compare the difference of AQP-1 fluorescence intensity between EC group and DHA group. Differences were considered significant at *p<0.05 (t-test via EC group). Scale bar=100 µm, AQP-1: aquaporin 1 (AQP-1); green, 4', 6-diamidino-2-phenylindole (DAPI): blue. NC: normal control group, EC: experimental control; reperfusion 24 hr after MCAO injury, DHA: DHA treatment and reperfusion 24 hr after MCAO injury.
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