Fig. 3. Immunochemical image for confirmation of preserved Claudin 5 expression by DHA treatment. (A) Immunochemical images showed that Claudin 5-positive cells (green) were obviously reduced in rat cortex of experimental control (EC) group whereas in DHA treatment group, its expression was preserved in cortex. (B) Claudin 5-positive cells were retained in rat striatum owing to DHA treatment. (C) The graph showed the percentage (%) of Claudin 5 positive cells to compare the difference of Claudin 5 fluorescence intensity. Differences were considered significant at *p<0.05 (t-test via EC group). Scale bar=100 µm, Claudin 5: green, 4', 6-diamidino-2-phenylindole (DAPI): blue. NC: normal control group, EC: experimental control; reperfusion 24 hr after MCAO injury, DHA: DHA treatment and reperfusion 24 hr after MCAO injury.
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