Fig. 7. Immunochemical image for confirmation of attenuated iNOS expression by DHA treatment. (A) Immunochemical images showed that iNOS positive cells (red) were considerably expressed in the EC group. The rat cortex in DHA treatment group showed reduced iNOS expression compared with EC group. (B) In DHA treatment group, iNOS positive cells were decreased in rat striatum. (C) The graph showed the percentage (%) of iNOS positive cells to compare the difference of iNOS fluorescence intensity. Differences were considered significant at **p<0.01 (t-test via EC group). Scale bar=100 µm, iNOS: inducible nitric oxide synthase (iNOS); red, 4', 6-diamidino-2-phenylindole (DAPI): blue. NC: normal control group, EC: experimental control; reperfusion 24 hr after MCAO injury, DHA: DHA treatment and reperfusion 24 hr after MCAO injury.
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