Fig. 3. COMP-Ang1 increase the Tie2/FAK interaction after translocation in hCB-EPCs. (A) Confocal images of Tie2, FAK, and AKT (red) with one of two location markers (CY or PM, green) and the DAPI nuclear marker (blue) with or without treatment of COMP-Ang1 in EPC. Yellow or cyan color indicate overlap between protein and the corresponding location markers. Scale bar, 200 µm. (B, C) Interactions between Tie2: FAK (left), Tie2: AKT (middle), or AKT: FAK (right) with or without COMP-Ang1 treatment in EPCs. A proximity ligation assay (PLA) was used to measure the population of close physical interactions. Red spots indicate the physical proximity of the corresponding protein pair. Scale bar, 200 µm. (B). Number of blobs (or interactions) per cell between Tie2 and FAK with or without COMP-Ang1 treatment in EPCs (C). Average number of "blobs" (spots or interactions) per cell for Tie2: FAK, Tie2: AKT, or AKT: FAK under the COMP-Ang1 treatment condition showing that the number of interacting pairs between Tie2: FAK increased dramatically, but interaction between Tie2: AKT and AKT: FAK decreased after COMP-Ang1 treatment. All values are the means±SEM. ***p<0.001. (D, E) Confocal images of Tie2, FAK, and AKT (red) with one of two location markers (CY or PM, green) and the DAPI nuclear marker (blue) in a rat model. (D) Densitometric analyses (E). Scale bar, 20 µm. All values are the means±SEM. ***p<0.001, **p <0.01, *p<0.05.
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