Fig. 4. COMP-Ang1 increases Tie2/FAK/ITG-mediated migration and tube formation. (A, C) Tube formation assay with COMP-Ang1 treated Tie2, FAK and AKT silencing (A). COMP-Ang1 (200 ng/mL) was incubated for 24 h followed by densitometric analyses (C). Scale bar, 100 µm. All values are the means±SEM. ****p <0.0001, *p<0.05. (B) HUVEC-DiI incorporation assays with COMP-Ang1 treated Tie2, FAK, and Akt silencing. COMP-Ang1 (200 ng/mL) was incubated for 24 h. Scale bar, 100 µm. (D) Scratch wound migration assay with COMP-Ang1 treated Tie2, FAK and AKT silencing. COMP-Ang1 (200 ng/mL) was incubated for 24 h. Scale bar, 50 µm. (E, F) The numbers of interacting pairs between ITGανβ3: ITGα4, ITGανβ3: ITGαV or ITGανβ3: ITGβ1 did not change after Tie2, FAK or AKT silencing. Interactions between ITGανβ3: ITGα4 (top), ITGανβ3: ITGαV (middle), or ITGανβ3: ITGβ1 (bottom) with/without COMP-Ang1 treatment in EPCs before or after Tie2 or FAK silencing. Red spots indicate the physical proximity of the corresponding protein pair. Scale bar, 200 µm. (E). Number of blobs (or interactions) per cell between ITGανβ3: ITGα4, ITGανβ3: ITGαV or ITGανβ3: ITGβ1 with/without COMP-Ang1 treatment in EPCs before or after Tie2 or FAK silencing (F). All values are the means±SEM. ***p<0.001.
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