Fig. 6. COMP-Ang1 increase angiogenesis by AKT/mTOR interaction. (A,B) Interaction between AKT and mTOR with/without COMP-Ang1 treatment in EPCs before or after Tie2 silencing. Red spots indicate the physical proximity of the corresponding protein pair. Scale bar, 200 µm. (A). Blobs showing interaction between AKT and mTOR with/without COMP-Ang1 treatment in EPCs before or after Tie2 silencing. The numbers of interacting pairs between AKT: mTOR no changed after Tie2 silencing (B). All values are the means±SEM. ***p<0.001. (C) Confocal images of ERK1/2, SAPK/JNK and mTOR (red) with DAPI of nucleus marker (blue) in rat model. Scale bar, 50 µm. (D, E) The relative protein expression levels of MAPK kinase with/without COMP-Ang1 treatment in EPC before or after AKT silencing or mTOR inhibition. Whole cell lysates (30 µg protein/lane) were subjected to Western blot analyses to determine the levels of ERK1/2, pERK1/2, p38, pp38, and SAPK/JNK, pSAPK/JNK. β-actin was used as an internal control for equal protein loading for each lane.
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