Fig. 3. miR-Let7A overexpression regulated Beclin 1, ATG3, LC3II protein levels in BV2 cells activated by LPS. (A~C) Western blotting showing the expression levels of Beclin 1 (A), ATG3 (B), and LC3II (C) and their quantifications in normal BV2 cells, BV2 cells transfected with miR-Let7A, BV2 cells treated with LPS (1 µg/ml), BV2 cells transfected with miR-Let7A and treated with LPS. miR-Let7A mimic was used at 20 nM and LPS was treated for 12 h. β-actin was used as a control. NC: normal control group, Let7A overexpression: miR-Let7A overexpression group, LPS: LPS treatment group. Data were expressed as mean±S.E.M, and each experiment included 3 repeats per condition. Differences were considered significant at *p<0.05, **p < 0.001.
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