Fig. 4. Immunocytochemical analysis of ATG3 expression in miR-Let7A-transfected BV2 microglia activated by LPS. (A, B) Immunocytochemical images showing ATG3 immunoreactivity (green) in normal BV2 cells, BV2 cells transfected with miR-Let7A, BV2 cells treated with miR-Let7A inhibitor, BV2 cells treated with LPS (1 µg/ml), BV2 cells transfected with miR-Let7A and treated with LPS. miR-Let7A mimic was used at 20 nM and LPS was treated for 12 h. NC: normal control group, miR-Let7A overexpression: Let7A overexpression group, LPS: LPS treatment group, LPS+miR-Let7A overexpression: LPS treatment plus miR-Let7A overexpression group, miR-Let7A inhibitor: miR-Let7A suppression group. Scale bar: 200 µm, 4', 6-diamidino-2-phenylindole (DAPI): blue, ATG3: green.
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