Fig. 2. Double immunofluorescence staining for the identification of GDF15-positive cells in the hippocampus of control (A~C) and KA-treated mice at 3 days after treatment (D, E). GDF15 was colocalized with the glial fibrillary acidic protein (GFAP) in the nuclei of positive cells in the CA3 region of KA-treated hippocampi (arrows), but not in neurons-like cells (arrowheads). Nuclear counterstaining was performed with DAPI (blue). Scale bar=20 µm.
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