Fig. 2. Immunohistochemical assays for the detection of alpha-synuclein protein. The figure shows the section of a salivary gland dyed with red fluorescence for neurofilament protein (A), the fluorescent DAPI with the genomic DNA in cell nuclei appears in blue (B) and the alpha-synuclein protein is detected by a monoclonal antibody and developed in green (C). The overlapping of images (D) highlights that the relative intensity of alpha-synuclein is larger on the left side of the gland in the image. The increase is not homogeneous in patients with the amplification of alpha-synuclein in mosaic. A:200×.
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