Fig. 5. MPP+-induced caspase activity is detected in primary cultures of DA neurons cultivated in 5.5 mM glucose. Primary cultures of DA neurons were prepared from the mesencephalon of ED 14 embryos. At 5 DIV, cells cultivated in the indicated concentrations of glucose were exposed to 3 µM MPP+ for 48 hr. (A) Cultures were subjected to a double immunofluorescent localization of tyrosine hydroxylase (TH) and active caspase 3, and photographed as described in Materials and Methods. Scale bar represents 50 µm. (B) Among TH-positive cells (300~400 cells) from two representative culture, means of percentage of the cells having a caspase-3 activity was determined.
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