Fig. 1. Discrepancy between MTT assay and actual cytotoxicity. (A) The degree of tetrazolium reduction was determined by MTT assay in primary glioblastoma cells (GBL-13, GBL-15) and glioblastoma cell lines (U87MG, U373MG). Four types of cells were treated with ethanol for 24 h, 48 h, and 72 h. The cell viability was measured by MTT assay. Values are expressed as mean and SD. *p<0.05 compared with the control group. (B) Microscopic images showing ethanol-induced morphological changes of the primary glioblastoma cells (GBL-13 and GBL-15) and glioblastoma cell lines (U87MG and U373MG). The images display only the control group and 5% ethanol-treated media group. All microscopic images took with 40x. (C) Trypan blue exclusion assay to determine ethanol-induced cytotoxicity in GBL-13, GBL-15, U87MG and U373MG. Values are expressed as mean and SD. *p<0.05 compared with the control group.
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